Fluorescent bioprobes for visualization of puromycin-sensitive aminopeptidase in living cells

Hiroki Kakuta; Yukiko Koiso; Kazuo Nagasawa; Yuichi Hashimoto

doi:10.1016/s0960-894x(02)00845-4

Fluorescent bioprobes for visualization of puromycin-sensitive aminopeptidase in living cells

Bioorg Med Chem Lett. 2003 Jan 6;13(1):83-6. doi: 10.1016/s0960-894x(02)00845-4.

Authors

Hiroki Kakuta¹, Yukiko Koiso, Kazuo Nagasawa, Yuichi Hashimoto

Affiliation

¹ Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Japan.

PMID: 12467622
DOI: 10.1016/s0960-894x(02)00845-4

Abstract

Non-peptide, small-molecular, non-competitive inhibitors of puromycin-sensitive aminopeptidase (PSA), that is, 3-(2,6-diethylphenyl)-2,4(1H,3H)-quinazolinedione (PAQ-22, 3) and its 1N-methyl analogue (MPAQ-22 4), were structurally modified to afford fluorescent bioprobes, ANTAQ (5) and DAMPAQ-22 (6). The cellular localization of PSA could be visualized by the use of these fluorescent bioprobes.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Aminopeptidases / analysis*
Aminopeptidases / antagonists & inhibitors
Fluorescent Dyes / chemical synthesis*
Fluorescent Dyes / chemistry
Humans
Inhibitory Concentration 50
Kinetics
Microscopy, Fluorescence
Puromycin / pharmacology
Quinazolines / chemical synthesis
Quinazolines / pharmacology
Tumor Cells, Cultured

Substances

Fluorescent Dyes
Quinazolines
Puromycin
Aminopeptidases